Development of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease-causing PirAB toxins in shrimp

Authors:
  • THTran Van Hieu
    Tran Van Hieu
  • DDDuong Ngoc Diem
    Duong Ngoc Diem
  • NHNguyen Phuoc Khai Hoan
    Nguyen Phuoc Khai Hoan
  • DYDo Thi Kim Yen
    Do Thi Kim Yen
  • HBHuynh Tuan Binh
    Huynh Tuan Binh
  • MDMai Hoang Thuy Dung
    Mai Hoang Thuy Dung
  • NTNguyen Thi Nguyet Thu
    Nguyen Thi Nguyet Thu
  • TTTran Linh Thuoc
    Tran Linh Thuoc
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Abstract
Acute hepatopancreatic necrosis disease (AHPND) is currently the most affected disease of shrimp farms worlwide As rapid and specific detection methods are essential to improve disease management, a lateral flow immunoassay (LFIA) was designed for the detection of PirAvp and PirBvp toxins in shrimp. In this study, polyclonal antibody based lateral flow immunoassay (LFIA) strip has been developed. The recombinant PirAvp and PirBvp toxin‐like proteins of Vibrio parahaemolyticus (VPAHPND) was used to immunize rabbits with the highest antibody titre after the third booster. This polyclonal rabbit antiserum was purified and used to develop a LFIA strip for the detection of AHPND pathogen. This test strip could detect PirAvp and PirBvp toxins up to 125 ng/mL by naked eye within 15 min. No cross-reactivity was observed with VPnon-AHPND. Furthermore, the sensitivity and specificity of LFIA were 94.0% and 98.0%, respectively. This LFIA strip could be used as a handy tool by the farmers and technicians in situ. ® 2023 Journal of Science and Technology - NTTU