Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture
Authors:
- K BrombergKBK Bromberg
- G TannisGTG Tannis
- B DaidoneBDB Daidone
- L ClarkeLCL Clarke
- M F SierraMSM F Sierra
- 1 December 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 22 (6) , 1071-1072
Abstract
Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. A blocking test confirmed those specimens as true positives (specificity, 100%).Keywords
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