Fourier Multiplexed Fluorescence Lifetime Imaging
Author:
- 31 July 2021
- book chapter
- Published by Springer Nature
- Vol. 2350, 157-172
Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a widely used functional imaging method in bioscience. Fourier multiplexed FLIM (FmFLIM), a frequency-domain lifetime measurement method, explores the principle of Fourier (frequency) multiplexing to achieve parallel lifetime detection on multiple fluorescence labels. Combining FmFLIM with a confocal scanning microscope allows multiplexed 3D lifetime imaging of cells and tissues. FmFLIM can also be integrated with the scanning laser tomography imaging method to perform 3D multiplex lifetime imaging of whole embryos and thick tissues.Keywords
This publication has 20 references indexed in Scilit:
- A practical implementation of multifrequency widefield frequency-domain fluorescence lifetime imaging microscopyMicroscopy Research and Technique, 2013
- Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscopeDevelopment, 2012
- Arginylation-dependent regulation of a proteolytic product of talin is essential for cell–cell adhesionJournal of Cell Biology, 2012
- Highly efficient 3D fluorescence microscopy with a scanning laser optical tomographOptics Express, 2011
- The Phasor Approach to Fluorescence Lifetime Imaging AnalysisBiophysical Journal, 2008
- Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence SpectroscopyAnalytical Chemistry, 2007
- Fluorescence lifetime imaging by time‐correlated single‐photon countingMicroscopy Research and Technique, 2003
- Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methodsJournal of Biomedical Optics, 2003
- Fluorescence lifetime imaging: multi-point calibration, minimum resolvable differences, and artifact suppressionCytometry, 2001
- Global Analysis of Fluorescence Lifetime Imaging Microscopy DataBiophysical Journal, 2000