Metabolic Engineering to Produce Sesquiterpenes in Yeast
Authors:
- 10 April 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 5 (10) , 1629-1632
Abstract
Presented here is a metabolically engineered yeast strain that produces sesquiterpenes. Epi-cedrol synthase expressed in a native yeast strain converted endogenous farnesyl pyrophosphate to 90 μg/L epi-cedrol. This system was genetically modified to increase foreign terpene yields to 370 μg/L. The best yields were obtained by overexpressing a truncated 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in a upc2-1 mating type a background. This system allows sufficient production to characterize novel sesquiterpene synthase genes.Keywords
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