Persistence of the pBR 322 plasmid in Escherichia coli K 12 grown in chemostat cultures
Authors:
- 1 July 1980
- journal article
- research article
- Published by Springer Nature in Antonie van Leeuwenhoek
- Vol. 46 (4) , 353-362
Abstract
Populations of a Escherichia coli K 12 strain, containing the vector plasmid p BR 322, were grown in chemostat culture under glucose- and phosphatelimited conditions. Resistance to tetracycline and ampicillin were lost after prolonged cultivation, resulting in the production of apparent plasmid-free populations which were more competitive than the original population. This competitiveness between plasmid-free and plasmid-containing populations was greatest in environments where the nutrient restriction was severe. Also during sequential subcultivation in batch cultures loss of plasmid was observed.Keywords
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