In Vitro Antioxidant Properties of Flavonoids and Polysaccharides Extract from Tobacco (Nicotiana tabacum L.) Leaves
Authors:
Open Access
- 21 September 2012
- Vol. 17 (9) , 11281-11291
Abstract
In the present study, antioxidant properties of flavonoids and polysaccharides from tobacco (Nicotiana tabacum L.) leaves were evaluated in several in vitro systems, e.g., scavenging activities on hydroxyl, superoxide anion, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals, and reducing power. Flavonoids showed much better activity than polysaccharides in scavenging activities on free radicals. When compared to the positive control, ascorbic acid, both showed weaker antioxidant potential. However, flavonoids possessed comparable superoxide anion, DPPH and ABTS radical scavenging abilities to ascorbic acid at high concentration (600 μg/mL). Meanwhile, it was found that flavonoids had prominent effects on the reducing power, which was equivalent to ascorbic acid, and was significantly higher than polysaccharides. These results clearly indicate that flavonoids are effective in scavenging free radicals and have the potential to be powerful antioxidants. Thus, tobacco leaves could be considered as a potential source of natural antioxidants for food, pharmaceutical, cosmetics or nutraceutical industries.Keywords
This publication has 31 references indexed in Scilit:
- Extraction of essential oil from discarded tobacco leaves by solvent extraction and steam distillation, and identification of its chemical compositionIndustrial Crops and Products, 2012
- Extraction, identification and characterization of the water-insoluble proteins from tobacco biomassJournal of the Science of Food and Agriculture, 2011
- Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leavesFood and Chemical Toxicology, 2011
- Isolation, characterization and immunological activity of a polysaccharide from the fruit bodies of an edible mushroom, Sarcodon aspratus (Berk.) S. Ito.Food Research International, 2011
- Recovery and preservation of phenols from olive waste in ethanolic extractsJournal of Chemical Technology & Biotechnology, 2010
- Isolation of carotenoids, flavonoids and polysaccharides from Lycium barbarum L. and evaluation of antioxidant activityFood Chemistry, 2010
- Identification of polyphenols in tobacco leaf and their antioxidant and antimicrobial activitiesFood Chemistry, 2008
- Antioxidant activity and phenolic compounds in 32 selected herbsFood Chemistry, 2007
- Functional Components in Soybean Cake and Their Effects on Antioxidant ActivityJournal of Agricultural and Food Chemistry, 2006
- Relationship between boron and phenolic metabolism in tobacco leavesPhytochemistry, 1998