New Frontiers in ADC Analysis: Advances in Chromatography, Electrophoresis, and Mass Spectrometry
Authors:
- Alain BeckAlain Beck
- Valentina D’aTriValentina D’aTri
- Olivier VerhoevOVOlivier Verhoev
- Lola Alez-MartinLALola Alez-Martin
- Léa LetissierLLLéa Letissier
- Arnaud DelobelArnaud Delobel
- Sandrine Fleury-SouverainSandrine Fleury-Souverain
- Yannis-Nicolas FrançoisYannis-Nicolas François
- Sarah CianféraniSarah Cianférani
- Rabah GahoualRabah Gahoual
- Davy GuillarmeDavy Guillarme
- Oscar Hernandez-AlbaOscar Hernandez-Alba
- 10 December 2025
- journal article
- review article
- Published by Springer Nature in The AAPS Journal
- Vol. 28 (1) , 34
Abstract
Antibody–drug conjugates (ADCs) are composed of a tumor-targeting mAb conjugated to a cytotoxic payload to enable the selective delivery of the cytotKeywords
This publication has 148 references indexed in Scilit:
- Kinetic Modeling of the Antibody Disulfide Bond Reduction Reaction With Integrated Prediction of the Drug Load Profile for Cysteine‐Conjugated ADCsBiotechnology and Bioengineering, 2024
- Imaged Capillary Isoelectric Focusing Coupled to High‐Resolution Mass Spectrometry (icIEF‐MS) for Cysteine‐Linked Antibody–Drug Conjugate (ADC) Heterogeneity Characterization Under Native ConditionElectrophoresis, 2024
- A protocol for setting‐up robust hydrophobic interaction chromatography targeting the analysis of intact proteins and monoclonal antibodiesAnalytical Science Advances, 2022
- Characterization of therapeutic mAb charge heterogeneity by iCIEF coupled to mass spectrometry (iCIEF–MS)Electrophoresis, 2022
- Switching positions: Assessing the dynamics of conjugational heterogeneity in antibody–drug conjugates using CE‐SDSElectrophoresis, 2022
- A novel microchip‐based imaged CIEF‐MS system for comprehensive characterization and identification of biopharmaceutical charge variantsElectrophoresis, 2019
- A Mass‐Spectrometry‐Based Modelling Workflow for Accurate Prediction of IgG Antibody Conformations in the Gas PhaseAngewandte Chemie International Edition, 2018
- Dynamics of Intact Immunoglobulin G Explored by Drift‐Tube Ion‐Mobility Mass Spectrometry and Molecular ModelingAngewandte Chemie International Edition, 2014
- Stability of IgG1 Monoclonal Antibodies in Intravenous Infusion Bags Under Clinical In-Use ConditionsJournal of Pharmaceutical Sciences, 2012
- Effects of Surfaces and Leachables on the Stability of BiopharmaceuticalsJournal of Pharmaceutical Sciences, 2011