Total Synthesis and Cloning of a Gene Coding for the Ribonuclease S Protein
Authors:
- 23 March 1984
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 223 (4642) , 1299-1301
Abstract
A gene for ribonuclease S protein, has been chemically synthesized and cloned. The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified. This flexibility facilitates tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior.Keywords
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