High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins
Authors:
- Tc HallTHTc Hall
- Ec CockingECEc Cocking
- 1 September 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 96 (3) , 626-633
Abstract
1. Inexpensive scintillation mixtures are described which enable the detection of as little as 40mumuc of (14)C in aqueous solution with an efficiency of counting of over 80%. 2. A rapid method for the counting of alkaline, acidic and neutral aqueous solutions of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. 3. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of (14)C-labelled proteins from plant tissues. 4. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.Keywords
This publication has 7 references indexed in Scilit:
- The effects of x-irradiation on the incorporation of amino acids into proteins of nuclei from rat thymus glandBiochemical Journal, 1964
- Binding sites as a limiting factor in amino acid incorporationArchives of Biochemistry and Biophysics, 1962
- Liquid-scintillation counting of 14C-labelled animal tissues at high efficiencyBiochemical Journal, 1961
- Liquid scintillation-counting for [14C]steroidsBiochemical Journal, 1959
- Vergleichende Untersuchungen zur quantitativen Eiweissbestimmung im Liquor und eiweissarmen L sungenJournal of Molecular Medicine, 1955
- UPTAKE OF RADIOACTIVE ALANINE IN VITRO INTO THE PROTEINS OF RAT LIVER FRACTIONSJournal of Biological Chemistry, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951