Continuous Production of Pectic Oligosaccharides in an Enzyme Membrane Reactor

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Abstract
The use of high methylated (60% to 66% methylation) and low methylated (8% methylation) pectin for the continuous production of pectic‐oligosaccharides in an ultrafiltration dead‐end membrane reactor was investigated. Substrate concentration (1% to 5% w/v), enzyme concentration (90 to 2700 Ul ‐1 ), and residence time (40 to 120 min) were the factors studied. Response surface methodology was used to model the processes. Two responses were determined: conversion of pectin(s) into product, and product dextrose equivalent values (DE). Mathematical models were fitted for each response. Conversions to product ranged from 67% to 99.7%. Product DE values increased with increasing residence time from 27 to 70. The average molecular‐weight distribution of the products obtained was 3.5 kDa when high methylated pectin was used as substrate and 3.8 kDa for low methylated pectin.

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