Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomes
Authors:
- S.V. KirillovSKS.V. Kirillov
- E.M. MakarovEME.M. Makarov
- Yu.P. SemenkovYSYu.P. Semenkov
- 27 June 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 157 (1) , 91-94
Abstract
The 30 S subunit contains 2 sites for tRNA binding (Phe‐tRNA, AcPhe‐tRNA, tRNAPhe OH) with the functional properties of D and A sites of the 70 S ribosome after attachment of 50 S subunit. The third (E) site specific for deacylated tRNA is introduced into 70 S ribosome by its 50 S subunit. The E‐site binding of tRNAPhe OH is not sensitive to either tetracycline and edeine, and practically codon‐independent. The affinity constant of tRNAPhe OH for the E site is 2–3 orders of magnitude lower than that for the D site.Keywords
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