Identification of a Glycosyltransferase from Mycobacterium marinum Involved in Addition of a Caryophyllose Moiety in Lipooligosaccharides

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Abstract
Deletion of Mycobacterium marinum MMAR2333 resulted in the loss of three of four subclasses of lipooligosaccharides (LOSs). The mutant was unable to extend an intermediate (LOS-II*) by addition of caryophyllose. These data and the predicted domain structure suggest that MMAR2333 is a glycosyltransferase involved in the generation of a lipid-linked caryophyllose donor.

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