Chloroplastic glutamine synthetase from tobacco leaves: a glycosylated protein

Authors:
  • FNFarida Nato
    Farida Nato
  • BHBartrand Hirel
    Bartrand Hirel
  • ANAimé Nato
    Aimé Nato
  • PGPierre Gadal
    Pierre Gadal
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Abstract
The amino acid and carbohydrate content of chloroplastic glutamine synthetase from tobacco leaves has been analysed. The enzyme subunit contanins 5% carbohydrate, mainly represented by glucosamine, galactosamine, glucose, galactose and mannose residues. The enzyme subunit displayed a single band of molecular mass 44000 Da after sodium dodecyl sulphate (SDS) electrophoresis. However, when isoelectrofocussing electrophoresis was performed, four subunits were evident differing by their charge. Furthermore, the four different subunits stained positively when tested with periodic acid Shiff reagent, showing that sugars and amino sugars were present within all the subunits.

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