Rapid typing of HLA-DQB1 DNA polymorphism using nonradioactive oligonucleotide probes and amplified DNA
Authors:
- 1 May 1991
- journal article
- Published by Springer Nature in Immunogenetics
- Vol. 33 (3) , 163-170
Abstract
The allelic sequence diversity at theHLA-DQBI locus has been analyzed by polymerase chain reaction (PCR) amplification and sequencing. Fifteen amino acid sequence-defined alleles (one previously unreported) and several silent nucleotide polymorphisms which subdivide these alleles have been identified. Here, we describe the specific amplification of theDQB1 second exon by several different PCR primer pairs and a simple and rapid typing procedure using a panel of 16 horseradish peroxidase (HRP)-labeled oligonucleotide probes capable of distinguishing theseDQBI alleles.Keywords
This publication has 22 references indexed in Scilit:
- HLA-DR and -DQ epitopes and monoclonal antibody specificityImmunology Today, 1989
- Avoiding false positives with PCRNature, 1989
- The Use of Non-Radioactive Oligonucleotide Probes to Analyze Enzymatically Amplified DNA for Prenatal Diagnosis and Forensic HLA TypingNature Biotechnology, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Structural characteristics of an antigen required for its interaction with Ia and recognition by T cellsNature, 1987
- Immunological Self, Nonself DiscriminationScience, 1987
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction1987
- Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probesNature, 1986
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- Binding of immunogenic peptides to Ia histocompatibility moleculesNature, 1985