The histone deacetylase HDA15 interacts with MAC3A and MAC3B to regulate intron retention of ABA-responsive genes
Preprint
Abstract
Histone deacetylases (HDAs) play an important role in transcriptional regulation involved in multiple biological processes. In this study, we investigate the function of HDA15 in abscisic acid (ABA) responses. Immunopurification coupled with mass spectrometry-based proteomics was used to identify the HDA15 interacting proteins. We found that HDA15 can interact with the core subunits of MOS4-Associated Complex (MAC), MAC3A and MAC3B. In addition, ABA enhances the interaction of HDA15 with MAC3B. hda15 and mac3a/mac3b mutants are ABA-insensitive in seed germination and hyposensitive to salinity. RNA sequencing (RNA-seq) analysis demonstrate that HDA15 and MAC3A/MAC3B not only affect the expression of ABA-related genes, but also regulate ABA-responsive intron retention (IR). Furthermore, HDA15 and MAC3A/MAC3B reduce the histone acetylation level of the genomic regions near ABA-responsive IRs. Our studies uncovered the role of histone deacetylation in ABA-mediated splicing regulation and identified that HDA15-MAC3A/MAC3B acts as an important regulation module to mediate splicing of introns in ABA responses. One Sentence Summary: HDA15 and MAC3A/MAC3B coregulate intron retention and reduce the histone acetylation level of the genomic regions near ABA-responsive retained introns.Keywords
This publication has 41 references indexed in Scilit:
- Structure of Arabidopsis HISTONE DEACETYLASE15Plant Physiology, 2020
- Identification of HDA15-PIF1 as a key repression module directing the transcriptional network of seed germination in the darkNucleic Acids Research, 2017
- The SET-Domain Protein SUVR5 Mediates H3K9me2 Deposition and Silencing at Stimulus Response Genes in a DNA Methylation–Independent MannerPLOS Genetics, 2012
- Chromatin remodelling in plant light signallingPhysiologia Plantarum, 2011
- Dynamic histone acetylation is critical for cotranscriptional spliceosome assembly and spliceosomal rearrangementsProceedings of the National Academy of Sciences, 2011
- The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicingBiochemical Society Transactions, 2010
- Acetylation by the Transcriptional Coactivator Gcn5 Plays a Novel Role in Co-Transcriptional Spliceosome AssemblyPLOS Genetics, 2009
- Roles of dynamic and reversible histone acetylation in plant development and polyploidyBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2007
- The Prp19p-Associated Complex in Spliceosome ActivationScience, 2003
- Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) Function as Transcriptional Activators in Abscisic Acid SignalingThe Plant Cell, 2002