Submerged culture of hamster kidney cells in a stainless steel vessel
Authors:
- 1 September 1965
- journal article
- research article
- Published by Wiley in Biotechnology and Bioengineering
- Vol. 7 (3) , 417-434
Abstract
This paper describes the design and operation of an industrial type culture system for the submerged growth of BHK 21 cells at a volume of 30 l. Using a modified Eagle's medium and with the culture controlled at pH 7.4 ± 0.05 and at a temperature of 35 ± 0.25°C., the cells grew from 0.5 × 106 to about 2.5 × 106 viable cells/ml. in 50 hr. at stirrer speeds of 330 and 460 rpm. The significance of certain aspects of design and operation of a culture system of this type is discussed.Keywords
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