Control of pancreatic amylase release in vitro: effects of ions, cyclic AMP, and colchicine

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Abstract
Summary: . The time course and concentration‐response relationship of amylase release from pieces of guinea‐pig pancreas in vitro in response to bethanechol and pancreozymin was determined. . Removal of Ca++ from the medium had no effect on basal amylase release but abolished the stimulating effect on release of bethanechol. . Elevation of the concentration of Mg++ in the medium increased basal amylase release and reduced the response to bethanechol. . Elevation of the concentration of K+ in the medium increased amylase release; this effect was blocked by a concentration of atropine which blocked also the response to bethanechol. . Cyclic AMP, dibutyryl cyclic AMP and theophylline failed to stimulate amylase release. Pancreatic cyclic AMP concentrations were found not to be increased by bethanechol, pancreozymin or an elevated concentration of K+ in the medium. . Colchicine had no effect on basal amylase release or the response to bethanechol or pancreozymin. . It is concluded that the coupling of stimulus to secretion involves ionic control but that neither cyclic AMP production nor microtubular mechanisms play a major role in controlling exocytosis in the pancreatic acinar cell. These findings are discussed in relation to the stimulus‐secretion coupling processes in other cells.

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