Application of Methionine Increases the Germination Rate of Maize Seeds by Triggering Multiple Phenylpropanoid Biosynthetic Genes at Transcript Levels

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Abstract
Methionine is an essential amino acid that initiates protein synthesis and serves as a substrate for various chemical reactions. Methionine metabolism plays an important role in Arabidopsis seed germination, but how methionine works in seed germination of maize has not been elucidated. We compared the changes in germination rate, the contents of methionine and folates, and transcriptional levels using transcriptome analysis under water or exogenous methionine treatment. The results indicate that the application of methionine increases seed germination rate (95% versus 70%), leading to significant differences in the content of methionine at 36 h, which brought the rapid increase forward by 12 h in the embryo and endosperm. Transcriptome analysis shows that methionine mainly affects the proliferation and differentiation of cells in the embryo, and the degradation of storage substances and signal transduction in the endosperm. In particular, multiple phenylpropanoid biosynthetic genes were triggered upon methionine treatment during germination. These results provide a theoretical foundation for promoting maize seed germination and serve as a valuable theoretical resource for seed priming strategies.
Funding Information
  • National Key Research and Development Program of China (2021YFF1000304, HAAS2023RCQD11, B23C1000417, WLSZD2020-001, CAAS-ZDRW202010)
  • Research Project of Hainan Academy of Agricultural Sciences (2021YFF1000304, HAAS2023RCQD11, B23C1000417, WLSZD2020-001, CAAS-ZDRW202010)
  • Hainan Yazhou Bay Seed Lab (2021YFF1000304, HAAS2023RCQD11, B23C1000417, WLSZD2020-001, CAAS-ZDRW202010)
  • Agricultural Science and Technology Innovation Program (2021YFF1000304, HAAS2023RCQD11, B23C1000417, WLSZD2020-001, CAAS-ZDRW202010)

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