The Effect of N Acetyl D-Galactosamine and Other Sugars on the Mitogenic Activity and Attachment of PHA to Tonsil Cells
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Open Access
- 1 June 1968
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 31 (6) , 747-757
Abstract
The effect of several simple sugars on the mitogenic and binding properties of PHA has been investigated. N acetyl D-galactosamine in concentration of 3-10 mg./ml. inhibited the incorporation of uridine-2-C14 and thymidine-2-C14 into the acid precipitable fraction of human tonsil cells stimulated by phytohemagglutinin. No inhibition, or a significantly lesser degree of inhibition was observed with N acetyl D-glucosamine or glucose. The binding of I131 labeled PHA to cells was decreased by NAGAL or EDTA. The EDTA inhibition, unlike the NAGAL inhibition was overcome by magnesium.Keywords
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