Expression disparity of brain-derived neurotrophic factor immunoreactivity and mRNA in ischemic hippocampal neurons
Authors:
- T.-H. LeeTLT.-H. Lee
- H. KatoHKH. Kato
- S.-T. ChenSCS.-T. Chen
- K. KogureKKK. Kogure
- Y. ItoyamaYIY. Itoyama
- 1 December 2002
- journal article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 13 (17) , 2271-2275
Abstract
We studied the spatial and temporal expression of BDNF immunoreactivity and mRNA in the hippocampal formation after transient forebrain ischemia in gerbils. Our study demonstrated that in the vulnerable CA1 neurons, there was a prolonged expression disparity between BDNF immunoreactivity and mRNA and the BDNF level was reduced, in contrast to the ischemia-resistant dentate gyrus neurons that showed transient expression disparity and maintained the BDNF level. This expression disparity of the neurotrophic factor may be related to delayed neuronal death. Double immunostaining showed that reactive astroglia and microglia could express BDNF, suggesting a possible involvement of these cells in the mechanism of neuronal survival after ischemia.Keywords
This publication has 22 references indexed in Scilit:
- Brain-Derived Neurotrophic Factor in Astrocytes, Oligodendrocytes, and Microglia/Macrophages after Spinal Cord InjuryNeurobiology of Disease, 2000
- Brain-Derived Neurotrophic Factor Improves Long-Term Potentiation and Cognitive Functions after Transient Forebrain Ischemia in the RatExperimental Neurology, 1999
- Temporal profile of nerve growth factor-like immunoreactivity after transient focal cerebral ischemia in ratsBrain Research, 1996
- Expresgions of nerve growth factor and p75 low affinity receptor after transient forebrain ischemia in gerbil hippocampal CA1 neuronsJournal of Neuroscience Research, 1995
- BDNF Protein Measured by a Novel Enzyme Immunoassay in Normal Brain and after Seizure: Partial Disagreement with mRNA LevelsEuropean Journal of Neuroscience, 1995
- Characterization of Nerve Growth Factor (NGF) Release from Hippocampal Neurons: Evidence for a Constitutive and an Unconventional Sodium‐dependent Regulated PathwayEuropean Journal of Neuroscience, 1995
- Brain-Derived Neurotrophic Factor Protects against Ischemic Cell Damage in Rat HippocampusJournal of Cerebral Blood Flow & Metabolism, 1994
- Reactive glia as rivals in regulating neuronal survivalGlia, 1993
- Temporal Profiles of Nerve Growth Factor β‐Subunit Level in Rat Brain Regions After Transient IschemiaJournal of Neurochemistry, 1992
- Molecular cloning and expression of brain-derived neurotrophic factorNature, 1989