UCP3 overexpression neutralizes oxidative stress rather than nitrosative stress in mouse myotubes
Authors:
- Cèlia Garcia-MartínezCGCèlia Garcia-Martínez
- Elisabet AmetllerEAElisabet Ametller
- Josep M. ArgilésJAJosep M. Argilés
- Sílvia BusquetsSBSílvia Busquets
- Francisco J. López-SorianoFLFrancisco J. López-Soriano
- 25 December 2008
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 583 (2) , 350-356
Abstract
The deleterious effects of oxidants on proteins may be modified by overexpression of uncoupling protein 3 (UCP3) in skeletal muscle cells exposed to hyperoxia or H 2 O 2 . UCP3 overexpression significantly attenuated the increase in protein carbonylation in response to hyperoxia and H 2 O 2 exposures. However, antioxidant enzyme content and activity (superoxide dismutases, peroxiredoxins, glutathione peroxidase‐I, and catalase) were reduced or not modified in UCP3‐overexpressing myotubes exposed to oxidants. Protein nitration increased in UCP3‐overexpressing cells exposed to hyperoxia, but not to H 2 O 2 . We conclude that protein oxidation rather than nitration is neutralized by UPC3 overexpression in mouse myotubes exposed to abundant reactive oxygen species.Keywords
This publication has 33 references indexed in Scilit:
- The effect of UCP3 overexpression on mitochondrial ROS production in skeletal muscle of young versus aged miceFEBS Letters, 2008
- Oxidative stress and disuse muscle atrophyJournal of Applied Physiology, 2007
- Antioxidants protect from atherosclerosis by a heme oxygenase-1 pathway that is independent of free radical scavengingJournal of Experimental Medicine, 2006
- Hyperoxia‐mediated oxidative stress increases expression of UCP3 mRNA and protein in skeletal muscleFEBS Letters, 2005
- Both oxidative and nitrosative stress are associated with muscle wasting in tumour‐bearing ratsFEBS Letters, 2005
- Activation of UCPs gene expression in skeletal muscle can be independent on both circulating fatty acids and food intakeFEBS Letters, 2005
- Oxidative damage and phospholipid fatty acyl composition in skeletal muscle mitochondria from mice underexpressing or overexpressing uncoupling protein 3Biochemical Journal, 2002
- The role of uncoupling proteins in pathophysiological statesBiochemical and Biophysical Research Communications, 2002
- Overexpression of UCP3 in cultured human muscle lowers mitochondrial membrane potential, raises ATP/ADP ratio, and favors fatty acid versus glucose oxidationThe FASEB Journal, 2001
- Skeletal muscle UCP2 and UCP3 gene expression in a rat cancer cachexia modelFEBS Letters, 1998