High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners
Authors:
- Lin CaoLCLin Cao
- Zhijun JiaZJZhijun Jia
- Gang WuGWGang Wu
- Teng LiTLTeng Li
- Fengxia LuFLFengxia Lu
- Zhaoxin LuZLZhaoxin Lu
- 1 June 2011
- journal article
- Published by Elsevier in Protein Expression and Purification
- Vol. 77 (2) , 185-192
Abstract
No abstract availableKeywords
This publication has 43 references indexed in Scilit:
- Functional expression of recombinant human ribonuclease/angiogenin inhibitor in stably transformed Drosophila melanogaster S2 cellsCytotechnology, 2008
- Inhibition of Human Pancreatic Ribonuclease by the Human Ribonuclease Inhibitor ProteinJournal of Molecular Biology, 2007
- A versatile ligation-independent cloning method suitable for high-throughput expression screening applicationsNucleic Acids Research, 2007
- Improving Solubility ofShewanellaoneidensisMR-1 andClostridiumthermocellumJW-20 Proteins Expressed intoEsherichiacoliJournal of Proteome Research, 2005
- Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble ProteinsJournal of Proteome Research, 2005
- Many Paths to Many Clones: A Comparative Look at High-Throughput Cloning MethodsGenome Research, 2004
- Escherichia coli maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fusedProtein Science, 1999
- Mechanism of Ribonuclease Inhibition by Ribonuclease Inhibitor Protein Based on the Crystal Structure of its Complex with Ribonuclease AJournal of Molecular Biology, 1996
- Crystallization and Preliminary X-ray Analysis of Porcine Ribonuclease Inhibitor, a Protein with Leucine-rich RepeatsJournal of Molecular Biology, 1993
- Expression of human placental ribonuclease inhibitor in Escherichia coliBiochemical and Biophysical Research Communications, 1989