RH 5849, a Nonsteroidal Ecdysone Agonist: Effects on a Drosophila Cell Line
Author:
- 22 July 1988
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 241 (4864) , 467-469
Abstract
The steroid molting hormone 20-hydroxyecdysone is the physiological inducer of molting and metamorphosis in insects. In ecdysone-sensitive Drosophila K c cells, the insecticide RH 5849 (1,2-dibenzoyl-1- tert -butylhydrazine) mimics the action of 20-hydroxyecdysone by causing the formation of processes, an inhibition of cell proliferation, and induction of acetylcholinesterase. RH 5849 also competes with [ 3 H]ponasterone A for high-affinity ecdysone receptor sites from K c cell extracts. Resistant cell populations selected by growth in the continued presence of either RH 5849 or 20-hydroxyecdysone are insensitive to both compounds and exhibit a decreased titer of measurable ecdysone receptors. Although it is less potent than 20-hydroxyecdysone in both whole-cell and cell-free receptor assays, RH 5849 is the first nonsteroidal ecdysone agonist.Keywords
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