DNA–polyfluorophore excimers as sensitive reporters for esterases and lipases
- 22 January 2010
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- Vol. 46 (8) , 1221-1223
Abstract
DNA-scaffolded oligodeoxyriboside fluorophores (ODFs) were used as the reporters in turn-on sensing of enzymatic bond-cleaving activity. A tetramer ODF of pyrene deoxynucleosides displayed high quenching efficiency when conjugated via ester linkages with a dabcyl quencher, and yielded large signal increases with several enzymes in vitro and in intact human cells.Keywords
This publication has 33 references indexed in Scilit:
- Polyfluorophore Excimers and Exciplexes as FRET Donors in DNABioconjugate Chemistry, 2009
- Polyfluorophore Labels on DNA: Dramatic Sequence Dependence of QuenchingChemistry – A European Journal, 2009
- Polyfluorophores on a DNA Backbone: A Multicolor Set of Labels Excited at One WavelengthJournal of the American Chemical Society, 2009
- Pyrene Excimer Signaling Molecular Beacons for Probing Nucleic AcidsJournal of the American Chemical Society, 2007
- Efficient Quenching of Oligomeric Fluorophores on a DNA BackboneJournal of the American Chemical Society, 2007
- Probing Phospholipase A2 with Fluorescent Phospholipid SubstratesChemBioChem, 2007
- Oligodeoxyfluorosides: strong sequence dependence of fluorescence emissionTetrahedron, 2007
- Fluorogenic Label for Biomolecular ImagingACS Chemical Biology, 2006
- Techniques to measure lipase and esterase activity in vitroMethods, 2005
- Novel Fluorogenic Substrates for Imaging β-Lactamase Gene ExpressionJournal of the American Chemical Society, 2003