EXPERIMENTAL TRANSMISSION OF THE DELTA VIRUS TO A HEPATITIS-B CHRONIC CARRIER CHIMPANZEE WITH THE DEVELOPMENT OF PERSISTENT DELTA CARRIAGE
Authors:
- HA FIELDSHFHA FIELDS
- S GOVINDARAJANSGS GOVINDARAJAN
- HS MARGOLISHMHS MARGOLIS
- CD SCHABLECSCD SCHABLE
- JW EBERTJEJW EBERT
- JE MAYNARDJMJE MAYNARD
- 1 February 1986
- journal article
- research article
- Vol. 122 (2) , 308-314
Abstract
A delta virus (DV) infection was experimentally transmitted to a hepatitis B chronic carrier chimpanzee. The infection was monitored by the examination of liver biopsy materials with the use of FITC-labeled human anti-delta IgG or by an indirect immunoperoxidase staining technique and by measurement of the serum alanine aminotransferase (ALT) activity. Three different antibody enzyme immunoassays (EIAs) were developed for assessment of the serologic response: (1) blocking assay, (2) IgM-specific capture assay, and (3) IgG-specific capture assay. An antigen-specific EIA was also developed for monitoring delta antigen in the serum. The results indicate that a superinfection with the DV produced a biphasic ALT response concomitant with the appearance of the delta antigen in biopsy materials. The persistence of these markers over the observation period (350 days) indicates the development of a persistent DV infection.Keywords
This publication has 16 references indexed in Scilit:
- Diagnosis of hepatitis A infection: Comparative specificity of IgM capture assays using antigens derived from tissue cultures and marmoset faecesJournal of Virological Methods, 1984
- Specificity and sensitivity of the IgM capture immunoassay: Studies of possible factors inducing false positive or false negative resultsJournal of Virological Methods, 1984
- Sensitive enzyme immunoassay for the detection of delta antigen and anti‐delta, Using serum as the delta antigen sourceJournal of Medical Virology, 1984
- Radioimmunoassay detection of igm antibodies to the hbv‐associated delta (δ) antigen: Clinical significance in δ infectionJournal of Medical Virology, 1982
- Experimental HBV and δ infections of chimpanzees: Occurrence and significance of intrahepatic immune complexes of HBcAg and δ antigenHepatology, 1981
- Purification of bovine conglutinin using pepsin digestionMolecular Immunology, 1981
- Intrinsically stable IgG aggregatesJournal of Immunological Methods, 1981
- Transmission of the Hepatitis B Virus-Associated Delta Antigen to ChimpanzeesThe Journal of Infectious Diseases, 1980
- Experimental infection of chimpanzees with antihemophilic (factor viii) materials: Recovery of virus‐like particles associated with non‐A, non‐B hepatitisJournal of Medical Virology, 1979
- Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers.Gut, 1977