Norepinephrine and epidermal growth factor: Dynamics of their interaction in the stimulation of hepatocyte DNA synthesis
Authors:
- 1 October 1985
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 125 (1) , 45-50
Abstract
Primary cultures of adult rat hepatocytes are stimulated to enter DNA synthesis by norepinephrine (NE). This stimulation is maximal if the hepatocytes are incubated with NE for more than 12 hr, beginning no later than 2–4 hr after the cells are first plated. After 24 hr in culture, hepatocytes are unresponsive to NE stimulation. A strong synerergistic interaction between NE and epidermal growth factor (EGF) may be observed in cultures incubated with both EGF and NE, or pretreated with NE, then exposed to EGF. This interaction may be related to the finding that NE, in similarity with other factors that enhance EGF stimulation, reduces binding at the EGF receptor during the first 24 hr in culture.Keywords
This publication has 27 references indexed in Scilit:
- Induction of DNA Synthesis in Cultured Rat Hepatocytes Through Stimulation of α 1 Adrenoreceptor by NorepinephrineScience, 1985
- Reduction of epidermal growth factor receptor affinity by heterologous ligands: Evidence for a mechanism involving the breakdown of phosphoinositides and the activation of protein kinase CBiochemical and Biophysical Research Communications, 1984
- Insulin inhibits the glucocorticoid‐mediated increase in hepatocyte EGF bindingJournal of Cellular Physiology, 1984
- Differential sensitivity of fibroblasts to epidermal growth factor is related to cyclic AMP concentrationJournal of Cellular Physiology, 1984
- The effect of dexamethasone on pyruvate kinase activity in primary cultures of hepatocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1983
- DNA synthesis in primary cultures of adult rat hepatocytes in a defined medium: Effects of epidermal growth factor, insulin, glucagon, and cyclic‐AMPJournal of Cellular Physiology, 1981
- Biologically active phorbol esters specifically alter affinity of epidermal growth factor membrane receptorsNature, 1979
- Inhibition of epidermal growth factor binding to surface receptors by tumor promotorsBiochemical and Biophysical Research Communications, 1979
- Hormonal factors and liver growthAdvances in Enzyme Regulation, 1978
- Insulin and non-suppressible insulin-like activity (NSILA-s): Evidence for separate plasma membrane receptor sitesBiochemical and Biophysical Research Communications, 1974