Application of Sedimentation Field-Flow Fractionation to Biological Particles: Molecular Weights and Separation

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Abstract
Sedimentation field-flow fractionation (SFFF) is an elution-centrifugation method which operates with an equilibrium solute layer thickness of the order of 10 μ. Retention is a function of molecular weight and density, thereby making it possible to obtain both fractionation and molecular weight values for particles and macromolecules. Equations for the characteristics of this method are given. Experimental data are obtained for bacteriophage T2 and compared with theoretical predictions. The molecular weight determined from these data are (227 ± 11) × 106 and (236 ± 7) × 106 for two different dilutions. Finally, it is noted that the potential speed and accuracy of the method are attractive, and are based largely on the small sedimentation layer thickness.

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