The Removal of Human Breast Cancer Cells from Hematopoietic CD34 + Stem Cells by Dielectrophoretic Field-Flow-Fractionation

Authors:
  • JYJun Yang
    Jun Yang
  • XWXiao-Bo Wang
    Xiao-Bo Wang
  • FBFrederick F. Becker
    Frederick F. Becker
  • PGPeter R.C. Gascoyne
    Peter R.C. Gascoyne
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Abstract
Dielectrophoretic field-flow-fractionation (DEP-FFF) was used to purge human breast cancer MDA435 cells from hematopoietic CD34+ stem cells. An array of interdigitated microelectrodes lining the bottom surface of a thin chamber was used to generate dielectrophoretic forces that levitated the cell mixture in a fluid flow profile. CD34+ stem cells were levitated higher, were carried faster by the fluid flow, and exited the separation chamber earlier than the cancer cells. Using on-line flow cytometry, efficient separation of the cell mixture was observed in less than 12 min, and CD34+ stem cell fractions with a purity > 99.2% were obtained. The method of DEP-FFF is potentially applicable to many biomedical cell separation problems, including microfluidic-scale diagnosis and preparative-scale purification of cell subpopulations.

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