Delayed Appearance of Labeled Protein in Isolated Nerve Endings and Axoplasmic Flow
Author:
- 6 November 1964
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 146 (3645) , 779-781
Abstract
Subcellular fractions of mouse brain were prepared by differential and sucrose density-gradient centrifugation at intervals up to 130 hours after intracerebral injection of C 14 -labeled leucine. The specific activity of the nerve ending fraction continued to rise during this period. Between 2 and 130 hours after injection there was a fivefold rise in the specific activity of the soluble protein prepared by lysing the nerve ending particles with water while the specific activity of the soluble protein from the whole homogenate concomitantly fell almost by a factor of three. The data are interpreted as being consistent with the appearance of protein in nerve endings by axoplasmic flow.Keywords
This publication has 5 references indexed in Scilit:
- The separation of synaptic vesicles from nerve-ending particles (‘synaptosomes’)Biochemical Journal, 1964
- Axonal migration of proteins in the central nervous system and peripheral nerves as shown by radioautographyJournal of Comparative Neurology, 1963
- ISOLATION OF SYNAPTIC VESICLES AND STRUCTURAL ORGANIZATION OF THE ACETYLCHOLINE SYSTEM WITHIN BRAIN NERVE ENDINGS*Journal of Neurochemistry, 1963
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Experiments on the mechanism of nerve growthJournal of Experimental Zoology, 1948