Hydrophilic Monodisperse Particles as Solid-Phase Material in Immunoassays: Comparison of Shell-and-Core Particles with Compact Particles

Authors:
  • KNK. Nustad
    K. Nustad
  • LJL. Johansen
    L. Johansen
  • JUJ. Ugelstad
    J. Ugelstad
  • TET. Ellingsen
    T. Ellingsen
  • ABA. Berge
    A. Berge
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Abstract
Hydrophilic monodisperse shell-and-core particles with a density of 1.07 were superior to heavier compact particles as a solid-phase material for immunoassays. The shell-and-core particles formed a semistable suspension for 24 h and were easily collected by centrifugation. The hydroxyl groups of the particles were activated with two sulfonyl chlorides. The most reactive one, tresyl chloride, gave rapid chemical coupling of antibodies, whereas tosyl chloride favored a rapid hydrophobic adsorption which was followed by slow chemical coupling. The solid-phase sheep antirabbit IgG made was used as a separation agent in several immunoassays and gave solid-phase primary antibodies by immunoadsorption of rabbit antibodies.