Real-time, high-throughput super-resolution microscopy via panoramic integration

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Abstract
We introduce super-resolution panoramic integration (SPI), an on-the-fly microscopy technique enabling instantaneous generation of subdiffractional images concurrently with scalable, high-throughput screening. SPI leverages multifocal optical rescaling, high-content sweeping, and synchronized linescan readout while preserving minimal post-processing and compatibility with epi-fluorescence settings. We demonstrate SPI for various subcellular and populational morphology, function, and heterogeneity. This versatile platform offers a practical pathway toward biological insights beyond traditional optical and computational constraints.
Funding Information
  • National Science Foundation (EFMA1830941, DBI2145235)
  • U.S. Department of Health & Human Services | National Institutes of Health (R35GM124846, R35-GM138030)
  • Georgia Tech Institute for Electronics and Nanotechnology, a member of the NSF-supported National Nanotechnology Coordinated Infrastructure

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