Reduced Representation Bisulfite Sequencing Library Preparation for Sperm DNA Methylation Analyses: Manual and Automated Methods
Authors:
- 10 April 2025
- book chapter
- Published by Springer Nature
- Vol. 2897, 531-561
Abstract
Among epigenetic modifications, DNA methylation is extensively studied in sperm, and variations in sperm DNA methylation patterns have been associated with fertility, sperm quality, and in vitro fertilization outcomes in several species. Reduced representation bisulfite sequencing (RRBS) is a cost-effective method that has been successfully applied to sperm. However, RRBS library preparation remains sensitive and labor-intensive and can be subjected to diverse sources of technical variation. In order to improve RRBS library preparation performance, as well as its reproducibility, we adapted the reference protocol and implemented it on a Hamilton pipetting automaton.Keywords
This publication has 12 references indexed in Scilit:
- Characterizing the properties of bisulfite sequencing data: maximizing power and sensitivity to identify between-group differences in DNA methylationBMC Genomics, 2021
- Accelerating Onset of Puberty Through Modification of Early Life Nutrition Induces Modest but Persistent Changes in Bull Sperm DNA Methylation Profiles Post-pubertyFrontiers in Genetics, 2020
- A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific featuresBMC Genomics, 2018
- Breeding animals for quality products: not only geneticsReproduction, Fertility and Development, 2015
- Function and information content of DNA methylationNature, 2015
- UpSet: Visualization of Intersecting SetsIEEE Transactions on Visualization and Computer Graphics, 2014
- Transgenerational Epigenetic Inheritance: Myths and MechanismsCell, 2014
- Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profilingNature Protocols, 2011
- Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterningNature, 2008
- Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysisNucleic Acids Research, 2005