Two Closely Related Subclass II SnRK2 Protein Kinases Cooperatively Regulate Drought-Inducible Gene Expression
Open Access
- 7 April 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 51 (5) , 842-847
Abstract
The subclass III group of SNF1-related protein kinase 2 (SnRK2) members is known to play an important role in ABA and osmotic stress signaling in Arabidopsis; however, the roles of other subclasses remain elusive. Here, we established a double mutant of SRK2C/SnR2.8 and SRK2F/SnRK2.7 to investigate the functions of subclass II SnRK2s. Microarray analysis suggested that subclass II SnRK2s regulate some drought-responsive genes involving ABA-responsive element binding transcription factors (AREB/ABF) and their targets, and quantitative reverse transcription– PCR confirmed that those genes were down-regulated significantly in srk2cf. This study indicates that subclass II SnRK2s also play important roles in drought stress signaling in Arabidopsis.Keywords
This publication has 21 references indexed in Scilit:
- Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in ArabidopsisProceedings of the National Academy of Sciences, 2009
- Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START ProteinsScience, 2009
- Regulators of PP2C Phosphatase Activity Function as Abscisic Acid SensorsScience, 2009
- Arabidopsis mutant deficient in 3 abscisic acid-activated protein kinases reveals critical roles in growth, reproduction, and stressProceedings of the National Academy of Sciences, 2009
- Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysisNature Protocols, 2007
- Phosphoproteomic identification of targets of the Arabidopsis sucrose nonfermenting-like kinase SnRK2.8 reveals a connection to metabolic processesProceedings of the National Academy of Sciences, 2007
- Identification of Two Protein Kinases Required for Abscisic Acid Regulation of Seed Germination, Root Growth, and Gene Expression in ArabidopsisThe Plant Cell, 2007
- Identification of Nine Sucrose Nonfermenting 1-related Protein Kinases 2 Activated by Hyperosmotic and Saline Stresses in Arabidopsis thalianaJournal of Biological Chemistry, 2004
- The Arabidopsis CDPK-SnRK Superfamily of Protein KinasesPlant Physiology, 2003
- The Homologous ABI5 and EEL Transcription Factors Function Antagonistically to Fine-Tune Gene Expression during Late EmbryogenesisThe Plant Cell, 2002