Citrus Reticulata Blanco Induces Apoptosis in Human Gastric Cancer Cells SNU-668
Authors:
- Hae Jeong ParkHPHae Jeong Park
- Mee Suk HongMHMee Suk Hong
- Hi-Joon ParkHPHi-Joon Park
- Min-Su KimMKMin-Su Kim
- Kang-Hyun LeemKLKang-Hyun Leem
- Jeung-Beum KimJKJeung-Beum Kim
- Youn Jung KimYKYoun Jung Kim
- Hye Kyung KimHKHye Kyung Kim
- 1 January 2005
- journal article
- Published by Taylor & Francis in Nutrition and Cancer
- Vol. 51 (1) , 78-82
Abstract
Citrus fruits have been known to reduce the proliferation of many cancer cells. The antiproliferative effects of Citrus reticulata Blanco (CR) extract, the immature tangerine peel, on human gastric cancer cell line SNU-668 were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, 4,6-diamidineo-2-phenylindole staining, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay, reverse transcription-polymerase chain reaction expressions of BCL-2, BAX and CASP-3 genes, caspase-3 activity, and immunocytochemistry of caspase-3. From the results of the morphological and biochemical assays, CR (50 μg/ml) increased the apoptosis of human gastric cancer cells with typical apoptotic characteristics, including morphological changes of chromatin condensation and apoptotic body formation. CR (50 μg/ml) reduced the expression of BCL-2, whereas the expression of BAX and CASP-3 was increased compared with the control group. Furthermore, caspase-3 activity and ca...Keywords
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