Detection of Epidermal Growth Factor Receptors and E‐Cadherins in the Basolateral Membrane of A431 Cells by Laser Scanning Fluorescence Microscopy
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Open Access
- 1 January 1991
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 82 (1) , 8-11
Abstract
We have examined the localization of epidermal growth factor (EGF) receptors and E‐cadherins in cultured A431 cells, an epidermoid carcinoma cell line, using a laser scanning fluorescence microscope. The fluorescence signals generated by monoclonal antihodies against EGF receptor and E‐cadherin were localized mainly in the cell‐cell contact sites, about 3 μm in width. When these areas for cell adhesion were scanned perpendicularly, fluorescence was detected from a point 4μ from the cell base for a distance of 8 μm towards the cell surface (about 16 μm from the cell base). These data suggest the subcellular localization of EGF receptors and E‐cadherins in the basolateral membrane of A431 cells.Keywords
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