Nocardia rayongensis sp. nov., isolated from Thai peat swamp forest soil

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Abstract
An actinomycete strain RY45-3T, isolated from a peat swamp forest soil in Rayong Province, Thailand, was characterized using a polyphasic approach. The strain belonged to the genus Nocardia based on morphological, physiological, biochemical and chemotaxonomic properties. Cell-wall peptidoglycan contained meso-diaminopimelic acid. The N-acyl group of muramic acid in cell-wall was glycolyl type. The diagnostic sugars in whole cell hydrolysates were galactose and arabinose. MK-8(H4 ω-cycl) was the major menaquinone. The major fatty acids were C16:0 and C18:1ω9c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidyl inositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 71 mol%. On the basis of 16S rRNA gene sequence similarity analysis, strain RY45-3T was closely related to Nocardia jiangxiensis JCM 12861T (98.9%), Nocardia nova JCM 6044T (98.8%) and Nocardia pseudobrasiliensis JCM 9894T (98.6%). The strain showed low level of DNA-DNA relatedness with N. jiangxiensis JCM 12861T, N. nova JCM 6044T and N. pseudobrasiliensis JCM 9894T (range from 3.6% to 55.3%). On the basis of the phenotypic characteristics and these results mentioned, this strain could be differentiated from the closely related type strains and represents a novel species of the genus Nocardia, for which the name Nocardia rayongensis sp. nov. (type strain RY45-3T =JCM 19832T= TISTR 2213T= PCU 334T) is proposed.

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