Development of a Method for Three-Dimensional Culturing of Human Mesenchymal Stem (Stromal) Cells Using a Cellulose Matrix

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Abstract
The development of cell-culture methods in 3D systems is important and necessary for the development of significant areas of modern cell biology. When cultivating in a 3D system, the tissue-specific architecture is reproduced, with the real microenvironment and cell behavior being more accurately recreated in vivo. Human mesenchymal stem/stromal cells (MSCs) are usually isolated and cultured as a monolayer 2D culture. In this work, we have developed a method for three-dimensional culturing and tissue-specific decidual differentiation of MSCs isolated from human endometrial tissue using a matrix obtained from a decellularized apple. Decellularized apple matrices have sufficient mechanical strength; are biocompatible, affordable, and easy to use; and have ample possibilities for surface modification. This system of cell culturing is suitable for studies using both confocal microscopy and flow cytometry. The model we have developed can become the basis for creating new cell products and tissue engineering structures for the needs of regenerative biomedicine.

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