The Radioimmunoassay of Pulmonary Surface Active Material in Sheep Lung

Authors:
  • RKRichard J. King
    Richard J. King
  • EGElias G. Gikas
    Elias G. Gikas
  • JRJohn Ruch
    John Ruch
  • JCJohn A. Clements
    John A. Clements
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Abstract
We have developed a radioimmunoassay for pulmonary surface active material in sheep lung using solid-phase techniques. The method is capable of measuring 1 to 10 picomoles of antigen with a reproducibility of about 15 per cent and can be carried out in the presence of a 100-fold excess of either serum or lipid. It appears to be specific for the antigen found in pulmonary surfactant.