Mercury induced the Accumulation of Amyloid Beta (Aβ) in PC12 Cells: The Role of Production and Degradation of Aβ
Authors:
Open Access
- 31 December 2013
- journal article
- Published by Springer Nature in Toxicological Research
- Vol. 29 (4) , 235-240
Abstract
Extracellular accumulation of amyloid beta protein (Aβ) plays a central role in Alzheimer's disease (AD). Some metals, such as copper, lead, and aluminum can affect the Aβ accumulation in the brain. However, the effect of mercury on Aβ accumulation in the brain is not clear. Thus, this study was proposed to estimate whether mercury concentration affects Aβ accumulation in PC12 cells. We treated 10, 100, and 1000 nM HgCl2 (Hg) or CH3HgCl2 (MeHg) for 48 hr in PC12 cells. After treatment, Aβ40 in culture medium increased in a dose- and time-dependent manner. Hg and MeHg increased amyloid precursor protein (APP), which is related to Aβ production. Neprilysin (NEP) levels in PC12 cells were decreased by Hg and MeHg treatment. These results suggested that Hg induced Aβ accumulation through APP overproduction and reduction of NEP.Keywords
This publication has 44 references indexed in Scilit:
- Low levels of copper disrupt brain amyloid-β homeostasis by altering its production and clearanceProceedings of the National Academy of Sciences, 2013
- In vitro Pb exposure disturbs the balance between Aβ production and elimination: The role of AβPP and neprilysinNeuroToxicology, 2011
- Modulation of γ-Secretase Reduces β-Amyloid Deposition in a Transgenic Mouse Model of Alzheimer's DiseaseNeuron, 2010
- Epigenetics, oxidative stress, and Alzheimer diseaseFree Radical Biology and Medicine, 2009
- Peripherally expressed neprilysin reduces brain amyloid burden: A novel approach for treating Alzheimer's diseaseJournal of Neuroscience Research, 2008
- Mercury in human brain, blood, muscle and toenails in relation to exposure: an autopsy studyEnvironmental Health, 2007
- Loss of Neprilysin Function Promotes Amyloid Plaque Formation and Causes Cerebral Amyloid AngiopathyThe American Journal of Pathology, 2007
- A Century of Alzheimer's DiseaseScience, 2006
- Pathways towards and away from Alzheimer's diseaseNature, 2004
- Common Structure of Soluble Amyloid Oligomers Implies Common Mechanism of PathogenesisScience, 2003