The effect of controlled feeding of glycerol on β‐galactosidase production by Escherichia coli in batch culture

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Abstract
A mutant of E. coli constitutive for β‐galactosidase has been grown in batch culture with the carbon source, glycerol, fed at various fixed rates to the culture. High feeding rates where growth was only slightly restricted gave final enzyme levels similar to those obtained in cultures where all the glycerol was added initially. Low feeding rates resulted in breakdown of the β‐galactosidase formed and gave reduced final levels of the enzyme.

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