Changes in Cell Adhesiveness and Physicochemical Properties of Cross-Linked Albumin Films after Ultraviolet Irradiation
- 22 December 2015
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 32 (1) , 203-210
Abstract
We discovered the unique cell adhesive properties of UV-irradiated albumin films. Albumin films prepared using a crosslinking reagent with epoxy groups maintained native albumin properties such as resistance to cell adhesion. Interestingly, the cell adhesive properties of films varied depending on UV irradiation time; specifically, cell adhesiveness increased until 2 h of UV irradiation, when the cell number attached to the film was similar to that of culture dishes, then cell adhesiveness decreased until 20 h of UV irradiation, after which the surface returned to the initial nonadhesive state. To elucidate the molecular mechanisms underlying this phenomenon, we examined the effect of UV irradiation on albumin film properties. The following changes occurred in response to UV irradiation: decreased alpha-helical structure and cleavage of albumin peptide bonds, and increased hydrophilicity and oxygen content of the albumin film surface. In addition, we found a positive correlation between the degree of cell...Keywords
Funding Information
- Ministry of Education, Culture, Sports, Science, and Technology (15K16334)
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