QUANTIFICATION OF RADICAL FORMATION IN OIL‐IN‐WATER FOOD EMULSIONS BY ELECTRON SPIN RESONANCE SPECTROSCOPY
Authors:
- 1 June 1999
- journal article
- Published by Wiley in Journal of Food Lipids
- Vol. 6 (2) , 149-158
Abstract
The tendency of formation of radicals in an oil‐in‐water food emulsion (a mayonnaise partly based on fish‐oil) was quantified by electron spin resonance (ESR) spectroscopy using the spin trapping technique. Among the spin traps examined, N‐tert‐butyl‐α‐phenylnitrone (PBN) was preferred for this type of product. Under mildly accelerated conditions (i.e. heating to 37C, a spin trap concentration of 2 mM ensured pseudo‐first order conditions for trapping of radicals in the assay for up to 36 h of thermal treatment. The resulting linearity between ESR‐signals and rate of radical formation allows a direct quantification by standardization against stable radicals dissolved in the product. The hydrophobic spin‐probe 12‐doxylstearic acid (in contrast to the hydrophilic 2,2,6,6‐tetramethyl‐l‐piperidinyl‐oxyl) gave a stable signal and a linearity existed between signal intensity and probe concentration.Keywords
This publication has 7 references indexed in Scilit:
- Oxidation in fish-oil-enriched mayonnaiseEuropean Food Research and Technology, 1999
- Electron Spin Resonance Spin Trapping Identification of Radicals Formed during Aerobic Forced Aging of BeerJournal of Agricultural and Food Chemistry, 1998
- Improvement for Oxidative Flavor Stability of Beer—Role of OH-Radical in Beer OxidationJournal of the American Society of Brewing Chemists, 1996
- Biological spin trapping methodology. III. Octanol-water partition coefficients of spin-trapping compoundsJournal of Biochemical and Biophysical Methods, 1996
- Spin Trapping: ESR parameters of spin adducts 1474 1528VFree Radical Biology and Medicine, 1987
- Spin trapping of superoxide and hydroxyl radical: Practical aspectsArchives of Biochemistry and Biophysics, 1980
- Shape of the hydrophobic barrier of phospholipid bilayers (Evidence for water penetration in biological membranes)The Journal of Membrane Biology, 1974