Association of plant K+in channels is mediated by conserved C‐termini and does not affect subunit assembly
Open Access
- 9 June 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 409 (2) , 166-170
Abstract
Inward rectifying potassium (K+ in) channels play an important role in turgor regulation and ion uptake in higher plants. Here, we report a previously unrecognized feature of these proteins: K+ in channel C‐terminal polypeptides mediate channel protein interactions. Using a C‐terminal fragment of potato guard cell K+ in channel KST1 in a yeast two‐hybrid screen two novel putative K+ in channel proteins (SKT2 and SKT3) were identified by interaction of their C‐termini which contained a conserved domain (KHA). Interactions were confirmed by Western blot‐related assays utilizing K+ in channel C‐termini fused to green fluorescence protein. Although deletion of the KHA‐domain abolished these interactions, K+ in currents were still detectable by patch‐clamp measurements of insect cells expressing these KST1 mutants, indicating that formation of a functional channel does not depend on this C‐terminal domain.Keywords
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