CD45-positive cells are not an essential component in cardiosphere formation

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Abstract
The cardiosphere (CS) is composed of a heterogeneous population of cells, including CD45+ cells that are bone marrow (BM)-derived. However, whether the CD45+ cells are an essential cell component in CS formation is unknown. The current study was undertaken to address this question. Cardiospheres (CSs) were harvested from 1-week post-myocardial infarction (MI) or non-MI hearts of C57BL/6 J mice. The process of CS formation was observed by timelapse photography. To analyze the role of BM-derived CD45+ cells in CS formation, CD45+ cells were depleted from populations of CS-forming cells by immunomagnetic beads. We recorded the number of CSs formed in culture from the same amount (105) of intact CS-forming cells, from CD45+-cell-depleted CS-forming cells and from CD45+ cells alone (n=6–9/cell type). CS-forming cells selectively aggregated together to form CSs by 35 h after plating. The depletion of CD45+ cells from CS-forming cells actually increased the formation of CSs (67±10 CSs/105 cells) compared with non-depleted CS-forming cells (51±6 CSs/105 cells, P+ cells from CS-forming cells did not form CSs in culture. Thus, BM-derived CD45+ cells including BM progenitors are neither necessary nor sufficient for CS formation.

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