A cellular and spatial map of the choroid plexus across brain ventricles and ages
Authors:
- Neil DaniNDNeil Dani
- Rebecca H. HerbstRHRebecca H. Herbst
- Naomi HabibNHNaomi Habib
- Joshua HeadJHJoshua Head
- Danielle DionneDDDanielle Dionne
- Lan NguyenLNLan Nguyen
- Cristin McCabeCMCristin McCabe
- Jin CuiJCJin Cui
- Frederick B. ShipleyFSFrederick B. Shipley
- Ahram JangAJAhram Jang
- Christopher RodmanCRChristopher Rodman
- Samantha J. RiesenfeldSRSamantha J. Riesenfeld
Preprint
Abstract
The choroid plexus (ChP), located in each brain ventricle, produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier, but is under-characterized. Here, we combine single cell RNA-Seq and spatial mapping of RNA and proteins to construct an atlas of each ChP in the developing and adult mouse brain. Each ChP comprises of epithelial, endothelial, mesenchymal, immune, neuronal, and glial cells, with distinct subtypes, differentiation states and anatomical locations. Epithelial, fibroblast, and macrophage populations had ventricle-specific, regionalized gene expression programs across the developing brain. Key cell types are retained in adult, with loss of developmental signatures and maturation of ventricle-specific regionalization in the epithelial cells. Expression of cognate ligand-receptor pairs across cell subtypes suggests substantial cell-cell interactions within the ChP. Our atlas sheds new light on the development and function of the ChP brain barrier system, and will facilitate future studies on its role in brain development, homeostasis and disease.Keywords
All Related Versions
- Published version: Cell, 184 (11), 3056.
This publication has 76 references indexed in Scilit:
- The sva package for removing batch effects and other unwanted variation in high-throughput experimentsBioinformatics, 2012
- The Cerebrospinal Fluid Provides a Proliferative Niche for Neural Progenitor CellsNeuron, 2011
- Development of the lateral ventricular choroid plexus in a marsupial, Monodelphis domesticaFluids and Barriers of the CNS, 2010
- The iron exporter ferroportin 1 is essential for development of the mouse embryo, forebrain patterning and neural tube closureDevelopment, 2010
- Transventricular delivery of Sonic hedgehog is essential to cerebellar ventricular zone developmentProceedings of the National Academy of Sciences, 2010
- Sonic hedgehog is required for vascular outgrowth in the hindbrain choroid plexusDevelopmental Biology, 2010
- Sonic hedgehog signaling regulates a novel epithelial progenitor domain of the hindbrain choroid plexusDevelopment, 2009
- The Glial Nature of Embryonic and Adult Neural Stem CellsAnnual Review of Neuroscience, 2009
- Role for Spi-C in the development of red pulp macrophages and splenic iron homeostasisNature, 2008
- Genome-wide atlas of gene expression in the adult mouse brainNature, 2006