Boronic Acid‐Mediated Activity Control of Split 10–23 DNAzymes

Authors:
Open Access
Publisher website
Google Scholar
Abstract
The 10–23 DNAzyme is an artificially developed Mg2+‐dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence‐specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg2+ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best‐performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.
Funding Information
  • Agence Nationale de la Recherche (ANR PCRI "TEMPLAR"-16-CE92- 0010-01)
  • Deutsche Forschungsgemeinschaft (DFG MU1396/11)

This publication has 56 references indexed in Scilit: