Production of Lycopene in the Non-Carotenoid-Producing Yeast Yarrowia lipolytica

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Abstract
The codon-optimized genes crtB and crtI of Pantoea ananatis were expressed in Yarrowia lipolytica under the control of the TEF1 promoter of Y. lipolytica . Additionally, the rate-limiting genes for isoprenoid biosynthesis in Y. lipolytica , GGS1 and HMG1 , were overexpressed to increase the production of lycopene. All of the genes were also expressed in a Y. lipolytica strain with POX1 to POX6 and GUT2 deleted, which led to an increase in the size of lipid bodies and a further increase in lycopene production. Lycopene is located mainly within lipid bodies, and increased lipid body formation leads to an increase in the lycopene storage capacity of Y. lipolytica . Growth-limiting conditions increase the specific lycopene content. Finally, a yield of 16 mg g −1 (dry cell weight) was reached in fed-batch cultures, which is the highest value reported so far for a eukaryotic host.

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