Figure S4 from Olaparib and Radiotherapy Induce Type I Interferon– and CD8+ T Cell–Dependent Sensitization to Immunotherapy in Pancreatic Cancer

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Abstract
Supplemental Figure 4. (A-D) Workflow of CD8+ T cell isolation. All T cells (excluding cycling T cells) were isolated (A) and CD8+, CD4+, and NK status was determined (B). CD8+ T cells were further isolated and clustered (C). CD8+ only status was confirmed via violin plots of Cd8a, Cd4, and Fcer1g (D). (E) UMAP projection of CD8+ T cell clusters split by treatment group. (F) Bar plot displaying the percentage of total cells sequenced identified as B cells. (G) GSEAplots of important pathways found to be enriched in CD8+ T cell comparisons following GSEA including interferon alpha response, interferon gamma response, IL2/STAT5 signaling, and G2M checkpoint for the comparison between olaparib+radiation+αPD-L1 CD8+ T cells and radiation+αPD-L1 CD8+ T cells.