Optimization Extraction, Purification and Antioxidant Activities of Polysaccharides from Penthorum Chinense Pursh

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Abstract
Penthorum chinense Pursh is a well-known traditional Chinese medicine, however, little attention has been paid to the polysaccharides of P. chinense (PCP). Therefore, it is great significance to investigate the characteristics and activities of PCP. In this study, response surface methodology was applied to optimize the extraction parameters of PCP. Then, two polysaccharides fractions (PCP-1 and PCP-2) were purified from PCP by using DEAE-52 chromatography, and there preliminary chemical properties and in vitro antioxidant activities were investigated. Results revealed that the optimum extraction conditions of PCP were identified as follow: ratio of water to raw material 20.6 mL/g; extraction time 3.5 h and extraction temperature 85°C. Based on these conditions, the maximum yield of PCP was 3.12% ± 0.19%. Purified fractions PCP-1 and PCP-2 were all acidic heteropolysaccharides, and PCP-1 mainly consisted of galactose and arabinose while PCP-2 mainly consisted of rhamnose, galacturonic acid, galactose and arabinose. Moreover, PCP-2 exhibited stronger scavenging activities against DPPH radical, hydroxyl radical and superoxide anion radical and chelating activity on Fe 2+ in vitro .

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