Assessing the Effects of Cadmium Stress on the Growth, Physiological Characteristics, and Metabolic Profiling of Rice (Oryza sativa L.) Using HPLC-QTOF/MS

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Abstract
Cadmium (Cd) pollution is an important environmental problem, as it is easily absorbed by plants and gradually accumulates in the human body through the food chain. This study aimed to elucidate the changes in the metabolic response of the rice cultivar “TanLiangYou215” under Cd stress. Rice was grown in soil culture at 0 (Control), 2 (Low group), and 10 (High group) mg/kg CdCl2 for 90 days. The ultrastructural, Cd content, antioxidant activity, and metabolic changes to the rice in different tissues were analyzed. Phenotypic characterization and ultrastructure showed that the rice roots and leaves were significantly damaged and plant growth was inhibited in the High group, while plant growth was promoted in the Low group. Overall, Cd showed a regularity of “low promotion and high inhibition”. Physiological indices revealed that rice was significantly affected by Cd stress. Compared to the Control, Cd stress resulted in higher antioxidant enzyme activities, and the Low group suffered less oxidative damage than the High group. Metabolomic studies revealed that Cd stress significantly altered the metabolic profiles of rice plants. Rice responded to Cd stress by upregulating amino acids and regulating related pathways, including alanine, aspartate and glutamate metabolism, and arginine and proline metabolism. The significant expression of flavonoids with antioxidant properties helped rice resist the oxidative damage caused by Cd accumulation in the root tissue; Cd stress significantly downregulated glycerophospholipid metabolism in the stem and leaf tissues, which affected the cellular activities in rice stem and leaf tissues. We investigated the effects of Cd stress on ultrastructure, antioxidant activity, and metabolic changes in different tissues of the rice variety TLY215. Moreover, the different tissues of TLY215 can regulate these metabolic pathways to resist Cd stress, which provides valuable insights into the response of TLY215 to different concentrations of Cd.
Funding Information
  • Guangxi Scholarship Fund of the Guangxi Education Department (Guijiaoren-2019-5, GuikeZY20198006, 21874032, GuikeAB17129003)
  • Central Government-Guided Local Science and Technology Development Project (Guijiaoren-2019-5, GuikeZY20198006, 21874032, GuikeAB17129003)
  • National Natural Science Foundation of China (Guijiaoren-2019-5, GuikeZY20198006, 21874032, GuikeAB17129003)
  • Guangxi Key Research Project (Guijiaoren-2019-5, GuikeZY20198006, 21874032, GuikeAB17129003)

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